Concentrated sulfuric acid is slowly added to form two layers. What amino acid gives a positive Hopkins Cole test?Ī protein solution is mixed with Hopkins Cole reagent, which consists of glyoxylic acid. Since arginine has a guanidine group in its side chain, it gives a red color with α-naphthol in the presence of an oxidizing agent like bromine solution. The amino acid that is detected in this test is arginine. What amino acid S is indicated by a positive Sakaguchi test?į) Sakaguchi Test: The Sakaguchi reagent is used to test for a certain amino acid and proteins. The test was developed by the French chemist Auguste Nicolas Eugene Millon (1812–1867). Which colour is developed in Millon’s test for tyrosine?Ī reddish-brown coloration or precipitate indicates the presence of tyrosine residue which occur in nearly all proteins. The violet color is a positive test for the presence of protein, and the intensity of the color is proportional to the number of peptide bonds in the solution. Which functional group is responsible for the visible positive result in biuret test?īiuret reagent is an alkaline solution of 1% CuSO4, copper sulfate. 7 What causes dehydration in the Hopkins Cole test?.6 What is the purpose of the Hopkin Cole test?.5 Which of the following is optically inactive amino acid?.4 Which chemical is used to test the proteins?.2 Which colour is developed in Millon’s test for tyrosine?.1 Which functional group is responsible for the visible positive result in biuret test?.
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